HPLC videos and instructions by Sigma-Aldrich

HPLC Column Fundamentals
Introduction to how solutes can interact with columns containing porous particles and stationary phases to result in separation. I'm listing the most helpful ones below

Nick the Hero: Boost HPLC Productivity
Key features and benefits of Ascentis Express columns and how to use them to boost HPLC lab productivity.

Ultra-high Performance using Fused-Core^® HPLC Columns (28 min)
Reviews the use of Ascentis Express columns with traditional instruments and with ultra-high pressure systems.

The Use of Alternate Selectivity in Reversed-Phase HPLC (43 min)
Chemical structure of various types of bonded phases.

Introduction to Ascentis HPLC Columns (23 min)
Provides an overview of the family of columns.

HPLC Instrument bandwidth and optimizing systems for high-performance (14 min)
Discusses sample dispersion in a HPLC system, and how it can be measured for a given flow rate. Lowering the instrument bandwidth is necessary to achieve the best performance from a high-efficiency column.

High-performance HPLC Fittings and Interconnects
Demonstrates the proper use of our exclusive fittings designed for use with ultra-high pressure and narrow-bore HPLC columns.