Basically remember the following two statements and then derive which type of inhibitor is being expressed.
- If one could overcome inhibitor by increase in [Substrate] then Vmax does not change
- If inhibitor changes affinity of enzyme to substrate then Km is changed (usually increased)
Competative inhibitor
- competes for active site
- can be overcome by increase in [S]
Uncompetative inhibitor
- Binds Enzyme-Substrate complex
- increases apparant Km (enzyme affinity to substrate)
- Decreases Vmax (cannot be overcome by additional [S])
Mixed inhibitor
- Has affinity to both free enzyme and ES complex
- Some have equal affinity = Non-competative inhibitor(rare)
- Cannot be overcome by increase in [S] so Vmax decrease
- For the most part affinity to substrate changes little to none(non-competative)
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